Derek talks about his blood work results using 100 mg Testosterone Propionate per week split into every day subq administrations

My Blood Work On 100 MG Testosterone Per Week (With Accurate LC/MS-MS)

I recently lowered my weekly TRT dose down to 100 mg Testosterone Propionate split into every day subcutaneous administrations with an insulin pin.

Micro-dosing every day results in the most stable blood serum concentrations possible, less aromatization, and more free testosterone.

Every day micro-dosing is the closest thing you can get to replicating what would otherwise be healthy endogenous secretion of testosterone.

While more stable blood serum concentrations could be achieved with every day administrations of a longer ester like Enanthate, I opt for short esters for a reason.

Why I Opt For Short Esters

The reason why I opt for short esters is because it gives me more leniency if I need to pull something in order to do an experiment for hair loss prevention, or for some health marker.

It allows me to change a variable very quickly, have the hormone clear my system, and then give me accurate data to work with for my next experiment much faster.

With long esters like Enanthate or Cypionate, if I wanted to do an experiment I would have to wait for a much longer hormone clearance period.

You might be wondering, “Why would you want your testosterone to clear the system?”

Well, some of my experiments are a bit unorthodox to say the least.

Why I Lowered My TRT Dose To 100 MG Testosterone Per Week

I've been trying to titrate my weekly TRT dose down to see what the lowest amount is that I can get away with without losing a high quality of life, good libido, good state of well-being, no brain fog, etc.

My oldest TRT regimen at the very beginning was 200 mg Testosterone Enanthate per week, which was way too much.

I was pretty much on a mini-cycle when I was using that much.

Since then, I've slowly pulled back my dosages, and have been really pushing the envelope this past year to see how low I can get the androgen load in my body without losing muscle or experiencing impaired physiological functions supported by androgens.

The reason why I'm doing this is because I believe that once you satisfy a therapeutic level of androgen receptor activation to support basic physiological functions, than any excess above and beyond that will likely just contribute to greater levels of organ stress than necessary.

Most notably in a cardiovascular context.

Basically, I want to minimize the likelihood of cardiovascular issues, while still maintaining as high of a quality of life as possible.

By titrating down, I can evaluate where things level out in terms of risk to reward.

Inaccurate Testosterone Blood Test Results

Before we get into my blood test results, I would not be doing my due diligence if I skipped over the opportunity to explain why it is absolutely critical that you get high sensitivity testing done whenever you assess your hormone profile.

As you may or may not know, the majority of men are not getting accurate blood test results.

A minority of men using Testosterone even get regular blood work, and of that minority, an even smaller minority are getting accurate lab testing.

If you get your Testosterone and Estradiol levels tested using the more primitive direct chemiluminescent immunoassays, you will not get accurate blood test results.

I urge you to get your hormone levels tested with liquid chromatography/tandem mass spectrometry (LC/MS-MS) moving forward if possible.

It is a more sensitive and specific method when measuring Testosterone and Estradiol concentrations when compared to immunoassay, and the difference in test results between the two methods are massive.

Next time you need to assess your hormone profile, I advise that you at least get the following high sensitivity hormone profile and add the sensitive assay Estradiol test to your cart on top of that.

High Sensitivity Hormone Profile

Sensitive Assay Estradiol

Direct ECLIA Vs. LC/MS-MS Blood Test Results

Direct ECLIA is stated to be “sufficiently sensitive and accurate” for screening for androgen dysfunctions in men.

The reality is, it is a rough way to assess deficiencies, but it is not a reliable way to accurately measure your hormone profile on TRT, and certainly not on supraphysiological doses of androgens.

If you aren't getting highly sensitive and specific testing done via LC/MS-MS, you will not get accurate blood test results.

I have proven this in my own blood work numerous times.

The following is one example of this.

Roche ECLIA estradiol test result Sensitive Assay Estradiol Test Result

These Estradiol test results came from the same tube of blood.

Night and day difference between the two results.

Standard blood test assays can't even differentiate between Testosterone in your body and entirely different anabolic steroids.

Free Testosterone Test Results While On NPP
My “Free Testosterone” Level When I Was On 0 MG Of Testosterone But 200 MG Of Nandrolone Phenylpropionate

Get high quality blood work when you get it.

If you don't, you will be completely in the dark when it comes to deciphering exactly what deficiencies and/or imbalances you have in your hormone profile.

My Total Testosterone On 100 MG Testosterone Propionate Per Week

This is what my total Testosterone level was on 100 mg Testosterone Propionate per week determined via LC/MS-MS.

Total Testosterone Level On 100 MG Testosterone Propionate Per Week (LC/MS)

465.4 ng/dL on a reference range of 264-916 ng/dL.

Frankly, I was a bit surprised.

I thought it was going to be closer to 600 to 700.

I was shooting for 700.

I don't want to be teetering into mini-cycle territory, but I also don't want to be walking around with very average levels.

465.4 isn't even average, it's low.

Keep in mind when you evaluate blood work that there should be a happy medium between quality of life vs. quantity.

There's a balance that I'm still trying to achieve, regardless of my shift in priorities over the past few years.

Ideally, I would have wanted to see a 700 ng/dL total testosterone level, or slightly higher.

A lot of people are going to say, “total testosterone doesn't matter, all that matters if free testosterone.”

Frankly, I think you would be best served by shooting for a high-normal free testosterone and a high-normal total testosterone level.

Just because free testosterone is the measurable bioavailable androgens we can use, it doesn't necessarily mean that total testosterone doesn't have some sort of impact on physiological functions when you're accounting for the SHBG bound, as well as albumin bound testosterone in the body.

There’s weakly bound testosterone in the body that may have some sort of physiological impact, and there may be other factors at play too that we are not yet even aware of that don't get accounted for when you only take free testosterone into account.

In addition, the free testosterone test can vary wildly.

It is notorious for being all over the map.

That’s why I like to take the result of total testosterone determined via LC/MS-MS as well as free testosterone into account.

I would just be more comfortable having them both on the high end of normal to be honest.

My Free Testosterone On 100 MG Testosterone Propionate Per Week

This is what my Free Testosterone (Direct) level was on 100 mg Testosterone Propionate per week.

Free Testosterone level on 100 MG Testosterone Propionate Per Week

19.6 pg/mL in a reference range of 9.3-26.5 pg/mL.

It’s not exactly where I'd want it, but it is certainly better than my total testosterone level.

My Estradiol, Sensitive On 100 MG Testosterone Propionate Per Week

Sensitive Assay Estradiol lab result based on the 100 mg testosterone adjustment

My Estradiol level determined via sensitive assay testing was 23.8 pg/mL in a reference range of 8.0-35.0 pg/mL.

My Estrogen sweet spot is around 30 pg/mL, so I evidently have some leeway to increase my weekly TRT dose.

Based on my blood work I probably have 25 mg or so of leeway where I can build up to higher (but still therapeutic) Testosterone levels.

I don't think there's going to be a significantly detrimental impact by me doing so.

If I do decide to increase my Testosterone dose, I would also increase the amount of aromatization in my body.

But, because my Estradiol is only at 23.8 pg/mL, that gives me a solid 11 pg/mL of leeway before I'm even at the top end of the reference range.

These blood test results justify the addition of another 25 mg Testosterone per week should I decide I want to boost my total and free testosterone levels a bit higher.

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30 thoughts on “My Blood Work On 100 MG Testosterone Per Week (With Accurate LC/MS-MS)”

  1. Interesting experiment. I didn’t know Test Prop could be administered subcutaneously, which changes the game for everyday injections.

    How long after your injection did you get blood drawn?

    Do you anticipate any unwanted consequences to your skin if you do this for an extended period of time?

    1. So derek if you do a pct and somehow able to replicate these normal hormonal value naturally, will you be able to maintain your muscle mass ? Does this also means that you are still under your genetic potential as you dont need super physiological amount of androgens to retain this physique ?

      1. Keep in mind, I haven’t been doing this low of a dose for years, this was a recent change. The amount of myonuclei I built via blasting are not below genetic baseline.

  2. Very interesting Derek. I’m going to speak to my doctor about LC/MS-MS testing. Not sure if my insurance will pay for it though.

    Please consider an article about the risks or lack of risk for someone healthy with no family history of health problems, running supraphysiological doses of testosterone weekly, year round. I mean like 300mg a week not 500. I ask because I only feel normal when I take around 300mg of test cyp a week. Anything less, I feel tired and don’t have much endurance or libido. Nothing else wrong with me, my bloodwork comes back excellent.

    1. On this blood test specifically, I didn’t measure it. It’s expensive to test these biomarkers constantly, and as long as I can see my free T, I am not overly concerned with my SHBG as my admin frequency is optimal, I have good E2 levels, I’m lean, and my diet is in check. If someone was trying to understand their full panel though, of course SHBG would be a critical marker to add in.

      1. I Got my bloods today from 175mg of Test E (pharma) weekly, daily subq.

        It showed 1448 ng/dl total, 23,7 pg/ml free and a SHBG of 72.1 nmol/L.

        Trying to get that SHBG down to get along with less Test, but atm im increasing to 30mg of Test E ed.

        Shows how different the needs of different people can be.

        1. Hey Patrick, when you say 175mg per week- is that incl esther or is that 175 test + the ethanate weight? Just interested because this very much matches my tests. I am injecting 175mg (incl esther) per week (split into two doses 1 each 3.5 days). My results are almost identical coming out at 1395 ng/dl total.

  3. Derek I’m wondering if you are taking an AI with this low dose of test prop . I’m doing something similar using 20 to 25 mg of test prop EOD.

    Also what are your thoughts on using masteron as an AI?


    1. No I’m not. If you need an AI to run physiologic TRT doses, then something is wrong imo. If you are going to use a DHT derivative as a makeshift anti-estrogen I would choose Proviron over Mast.

  4. How do you feel on this dose? I’m assuming this is split to ~15mg daily? Depending on your shbg even daily prop can have wide variations. It hard to say where your peak is. You’re using pharma grade correct? Also a small subset of guys don’t absorb subq properly. I tried it with cyp myself and my test levels were several hundred point lower on the same dose.

  5. What was your total cholesterol and hdl and ldl?

    Im curious im on 150mg test e every week trt. And my ldl was 4.0 (.. but it would be better to have it under 3. And my hdl were slighlt lowered 0.8.. ( 0,8-2,1 mmol/l)

    Thinking of lowering my dosage to 125 mg and see if it helps. Eating a lot of fiber, and almost no saturated fats.. only trace from peanutt butter, salmon and eggs.

    Just curious what your cholesterol looks like, and what have you done/do to lower the bad and raise the good. Do you think its a good idea to dont eat eggs?

    1. For this blood test in particular:

      Cholesterol, Total 123 mg/dL
      Triglycerides 35 mg/dL
      HDL Cholesterol 39 mg/dL
      VLDL Cholesterol Cal 7 mg/dL
      LDL Cholesterol Calc 77 mg/dL

  6. I recently ran both series of test from labcorp.
    Estradiol sensitive 39.3 (8.0-35.0) a little high
    Estradiol (eclia) 50.4 (7.6-42.6) same a little high. Not much difference
    Testosterone serum 900 (264-916)
    Free direct 20.8 (7.2-24.0)
    This is running Testosterone Cypionate 60mg IM every 60 hrs (week split evenly 3 times total 180 mg a week) . I was tested 65 hours after last pin . I am going to go down to 150 mg a week. What do you think my number would be 12 hrs after I pin ?

  7. Hi Derek,

    When do you inject prop? Morning, mid day, evening?

    I’ve been injecting late evening and I’ve noticed that if I inject mid day I get bit awake at the bed time. Curious if anyone else experience that.

    Also if you were to switch to cypio or enan how would you split your dosage?

    I’ve been doing prop for past 3 years, currently 30 EOD and was considering change to 15 ED or different ester… I don’t mind doing prop ED if it will help me feeling better but at the same time I am curious about cypio or enan and less frequent pins.

    Great content!

  8. Hi Derek what are your thoughts on having to backfill DHEA, pregnenolone and progesterone while on TRT?

    Do you supplement any of those ?


  9. hey Derek, 51 yrs of age, 35 yrs bodybuilding and my TRT consist of test e 100mg, dros e 80mg, tren e 20mg per 1 ml per wk. i take .5ml on mon and .5ml on thurs. the dros gives a very nice boost of free test and a nice decrease of SHBG which i feel is very important just as much a decent high total t . the low dose tren is used primarily as the function of a SARM. i use 12.5mg aromasin because when you get a high free t response this will aromatize and taken account for the hcg as well. cabaser .5mg per wk to reduce prolactin some and this increases natural test levels. i did the ed and eod of micro doses subq simply for more stable levels and less E2 but pinning TRT ed or eod when i pin gh ed it gets old. lipid profile and hepatic function on my labs are good but i do add some hi anabolics from time to time and of course there goes the lipids and hepatic function. this TRT i put together myself helps hold me on to a real good shape at 250lbs+ , ya to many they will say this isnt a TRT with all the ancillaries but from what i use to run just a few months ago this is TRT! ok, well from time to time i will run a hi anabolic with it for 8wks. cant totally quit after 30+ decades!

  10. Hey Derek,

    one of the reasons why you saw such low blood serum conc of TOTAL test on a 100mg dose could also be the fact that you weigh in at 220-225 lbs

    with considerably more blood than a smaller guy who weighs in at say 180-190lbs
    the conc of your drug is going to be way less than what it shows up in his test, say 750-800 nd/dl in his case at the exact same 100mg dose

    will show up as 450-500 ng.dl in your body where it was diluted due to the 30% extra blood in your body

  11. Hi Derek,
    Fertility aside, are there any reasons one would NEED HCG to get dialed in on trt? For libido, erections, mood, proper levels of downstream hormones, etc. Would a T only protocol be best?

  12. Hi Derek,

    when it comes to SHBG, LH, FSH, I could’t find any labs offering LC-MS/MS.

    Seems like the next best alternative here is ECLIA, am I correct?

    Best, Chris

  13. so interesting; thank you for your intel and knowledge; I have a favor to ask, could you tell me about my testosterone situation please. i have a broken femur for 21 months now and really take long to consolidate; I wondered if my testo level could be a reason because I am 38 years old and already got my share of big injuries:

    My country doesn t allow this TRT sitation but i could make the test in Brasil to have an idea of my testosterone /

    Testosterona Livre e BiodisponívelMaterial :
    Sangue Metodo :Conforme descrito por Vermeulen et al., J Clin Endocrinol Metab 1999.
    Testosterona Livre 0,319 nmol/L VR:Homens (17 a 40 anos): 0,118 a 0,854 nmol/L

    Testosterona Livre (Porcentagem) 2,1% VR:Homens (> 18 anos): 1,7 a 2,3 %

    Testosterona Biodisponível 7,80 nmol/L VR:Homens (Acima de 18 anos): 4,55 a 23,67 nmol/L

    Testosterona Biodisponível (Porcentagem) 58,2% VR:Homens (> 18 anos): 37,0 a 47,0 %
    Índice Androgênico Livre (FAI / IAL) 57,3 VR:Homens: 14,8 a 94,8

    Testosterona Total 386,3 ng/dL Material :SangueMetodo :Quimioluminescência (CLIA)VR :Homens adultos: 200 a 1100 ng/dL

    It s in portugues but I know you inderstand the result:

    do you think a trt dosage with propionate everyday would help restore T level and help bone construction:

    thank you so much Dereck, you are an exemple


  14. Edward John Cody

    Are you still doing TRT at 125mg/week? With that dose do you feel any strength benefits?
    @ 100mg/wk you were in the middle of the range – without TRT you would be low normal possibly? Or even lower. So this makes you a true candidate for TRT? Is this genetic? Or from Blasting?

    (I’m trying to fit your answers to my own experiences) Thanks buddy..

  15. Are you still doing TRT at 125mg/week? With that dose do you feel any strength benefits?
    @ 100mg/wk you were in the middle of the range – without TRT you would be low normal possibly? Or even lower. So this makes you a true candidate for TRT? Is this genetic? Or from Blasting?

    (I’m trying to fit your answers to my own experiences) Thanks buddy..

  16. I’d like to transition from weekly IM to daily SubQ
    But, the problem I see is the injection apparatus – they are too large for smaller doses.

    Current setup for IM

    dose size is 0.4ml (of 200mg CYP) weekly into thigh muscle using 1ml syringe ; 0.4ml is 40% of the syringe capacity. So it can be kind of accurate. I like the luer lock needles, since they are easy to change out. I use a thicker needle to extract from vial. Swap the needle with a fresh one for IM injection. This preserves the quality of the needle tip. Luer lock needles work great.

    To go to daily SubQ

    Dose size is 15mg – daily, which works out to 0.075ml. I can find correct needle sizes for SubQ, but the problem is the small amount in a 1ml syringe ! If I went to 100mg CYP, then the dose amount is 0.15ml. That’s only 15% of the syringe capacity. WAY TOO LOW !

    Weird back-filling method

    I saw a weird video where the guy explained how he took an insulin needle/syringe, where he took the plunger out and injected the extracted fluid into the insulin syringe. He called this back-filling. That’s a horrible way to fill a syringe! I hate this.

    while there are 0.3ml insulin syringes, the needles are small and if you use it to puncture the vial, you risk damaging the gentile tip of the needle. 0.3ml syringe size would be perfect for me.

    There appears to be no good syringe that is accurate for daily SubQ injections of small doses ?

    It doesnt look like insulin syringes can be filled properly from a vial of testosterone CYP??

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